D2650
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D2650
Sigma
Dimethyl sulfoxide
≥99.7%, Hybri-Max™, sterile-filtered, hybridoma tested
| Synonym: | DMSO, Methyl sulfoxide |
| CAS Number: | 67-68-5 |
| Linear Formula: | (CH3)2SO |
| Molecular Weight: | 78.13 |
| Beilstein Registry Number: | 506008 |
| EC Number: | 200-664-3 |
| MDL number: | MFCD00002089 |
| PubChem Substance ID: | 24893703 |
Description
| Frequently Asked Questions | Live Chat and Frequently Asked Questions are available for this Product. |
| Application | DMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS). |
| Caution | Supercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product. |
| Packaging | 5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle |
| Legal Information | Hybri-Max is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co. |
Properties
| grade | Hybri-Max™ |
| vapor density | 2.7 (vs air) |
| vapor pressure | 0.42 mmHg ( 20 °C) |
| sterility | sterile-filtered |
| assay | ≥99.7% |
| autoignition temp. | 573 °F |
| expl. lim. | 42 %± , 63 °F |
| total impurities | Endotoxin, tested |
| refractive index | n |
| bp | 189 °C(lit.) |
| mp | 16-19 °C(lit.) |
| density | 1.10 g/mL(lit.) |
| suitability | hybridoma tested |
Safety
| Personal Protective Equipment | Eyeshields, Gloves, half-mask respirator (EU), half-mask respirator (US), multi-purpose combination respirator cartridge (US) |
| WGK Germany | 1 |
| RTECS | PV6210000 |
| UEL | 42.00%(V) |
| LEL | 3.50%(V) |
| Flash Point(F) | 188.6 °F |
| Flash Point(C) | 87 °C |
Related Products
| Related product | D1435, Dimethyl sulfoxide |
References
| reference | Pegg DE., Principles of cryopreservation Methods Mol. Biol. 368, 39-57, (2007) Abstract |
| Kleman MI, et al., Optimal conditions for freezing CHO-S and HEK293-EBNA cell lines: influence of Me2SO, freeze density, and PEI-mediated transfection on revitalization and growth of cells, and expression of recombinant protein Biotechnol. Bioeng. 100, 911-922, (2008) Abstract | |
| Merck | Merck 13,3285 |
| Beilstein | Beil. 1,IV,1277 |
| Fieser | Fieser 1,296 / Fieser 2,157 / Fieser 3,119 / Fieser 4,192 / Fieser 5,263 / Fieser 6,225 / Fieser 7,133 / Fieser 8,198 / Fieser 9,184 / Fieser 9,189 / Fieser 10,166 / Fieser 11,214 / Fieser 12,212 / Fieser 13,124 / Fieser 16,149 |
| Aldrich MSDS 1, 1308:D / Corp MSDS 1 (2), 2440:C / FT-IR 2 (1), 1492:B / FT-IR 1 (1), 879:A / FT-NMR 1 (1), 1417:A / IR-Spectra (3), 529:A / NMR-Reference 2 (2), 781:A / RegBook 1 (1), 1043:A / Sax 6, 1201 / Structure Index 1, 162:A:1 / Vapor Phase 3, 831:A |
