D2650

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D2650

Sigma

 

Dimethyl sulfoxide

≥99.7%, Hybri-Max™, sterile-filtered, hybridoma tested

Synonym:DMSO, Methyl sulfoxide
CAS Number:67-68-5
Linear Formula:(CH3)2SO
Molecular Weight:78.13
Beilstein Registry Number:506008
EC Number:200-664-3
MDL number:MFCD00002089
PubChem Substance ID:24893703

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Description

Frequently Asked QuestionsLive Chat and Frequently Asked Questions are available for this Product.
Related KeywordsDMSO, Methyl sulfoxide, (methanesulfinyl)methane, dimethyl sulphoxide, Deltan, Demeso, Demasorb, DMS-90, DMS-70, dimethylsulfoxid, dimethyli sulfoxidum, dimetyylisulfoksidi, Dimethylsulfoxyd, dimetilsulfossido, dimethili sulfoxidum, dimetilsulfóxido
ApplicationDMSO is a polar aprotic solvent used in chemical reactions, in polymerase chain reactions (PCR) and as a cryoprotectant vitrification agent for the preservation of cells, tissues and organs. DMSO is used in cell freezing media to protect cells from ice crystal induced mechanical injury. It is used for frozen storage of primary, sub-cultured, and recombinant heteroploid and hybridoma cell lines; embryonic stem cells (ESC), and hematopoietic stem cells. DMSO is frequently used in the combinations with BSA or fetal bovine serum (FBS).
CautionSupercools easily and remelts slowly at room temperature. Solidified product can be re-liquified by warming to room temperature without detriment to the product.
Packaging5 mL and 10 mL in flame sealed ampules, 100 mL in amber bottle
Legal InformationHybri-Max is a trademark of Sigma-Aldrich Biotechnology LP and Sigma-Aldrich Co.

Properties

gradeHybri-Max™
vapor density2.7 (vs air)
vapor pressure0.42 mmHg ( 20 °C)
sterilitysterile-filtered
assay≥99.7%
autoignition temp.573 °F
expl. lim.42 %± , 63 °F
total impurities Endotoxin, tested
refractive indexn20/D 1.479(lit.)
bp189 °C(lit.)
mp16-19 °C(lit.)
density1.10 g/mL(lit.)
suitabilityhybridoma tested

Safety

Personal Protective EquipmentEyeshields, full-face respirator (US), Gloves, multi-purpose combination respirator cartridge (US), type ABEK (EN14387) respirator filter
WGK Germany1
RTECSPV6210000
UEL42.00%(V)
LEL3.50%(V)
Flash Point(F)188.6 °F
Flash Point(C)87 °C

References

referencePegg DE., Principles of cryopreservation Methods Mol. Biol. 368, 39-57, (2007) Abstract
 Kleman MI, et al., Optimal conditions for freezing CHO-S and HEK293-EBNA cell lines: influence of Me2SO, freeze density, and PEI-mediated transfection on revitalization and growth of cells, and expression of recombinant protein Biotechnol. Bioeng. 100, 911-922, (2008) Abstract
MerckMerck 13,3285
BeilsteinBeil. 1,IV,1277
FieserFieser 1,296 / Fieser 2,157 / Fieser 3,119 / Fieser 4,192 / Fieser 5,263 / Fieser 6,225 / Fieser 7,133 / Fieser 8,198 / Fieser 9,184 / Fieser 9,189 / Fieser 10,166 / Fieser 11,214 / Fieser 12,212 / Fieser 13,124 / Fieser 16,149
 Aldrich MSDS 1, 1308:D / Corp MSDS 1 (2), 2440:C / FT-IR 2 (1), 1492:B / FT-IR 1 (1), 879:A / FT-NMR 1 (1), 1417:A / IR-Spectra (3), 529:A / NMR-Reference 2 (2), 781:A / RegBook 1 (1), 1043:A / Sax 6, 1201 / Structure Index 1, 162:A:1 / Vapor Phase 3, 831:A